Júnior started his career in Santo André. In 2010, he transferred to Ituano. After a good performance in early 2011, he had his federative rights purchased to Avaí and was loaned to Paraná Clube immediately. In the middle of 2011 season, Avaí called back Junior Urso to play in the Série A.[2] He made his debut for Avaí in the game which Avaí loses to Grêmio in Ressacada.[3] In the end of 2011, he was hired with Coritiba.
Following contract disputes with Shandong Luneng, the Chinese club loaned Urso back to Brazil where he played the 2016 season with Atlético Mineiro. The loan fee was a reported US$2 million (R$7.2 million).[5]
Guangzhou R&F
On 9 January 2017, Júnior moved to fellow Super League side Guangzhou R&F.[6]
Corinthians
On 4 February 2019, Júnior Urso returned to Brazil, signing with Corinthians after Guangzhou terminated his contract.[7] He made his debut for the team on 17 February against São Paulo FC in the 2019 Campeonato Paulista, a competition Corinthians would go on to win.[8] Three days later he scored his first goal for the club in a 4–2 win over Avenida in the Copa do Brasil.[9]
Orlando City
On 6 January 2020, Corinthians confirmed the sale of Urso to MLS team Orlando City ahead of the 2020 season, reportedly for a fee of $900k.[10][11] Orlando announced his arrival the following week.[12] Urso made his debut for the team in the season opener, captaining the side in place of the suspended Nani as Orlando earned a 0–0 draw against Real Salt Lake.[13] On 29 August 2020, Urso scored the opener in a 3–1 win over Atlanta United, Orlando's first ever win in the rivalry's history.[14] On 10 November 2022, Orlando City and Urso mutually agreed to terminate his contract, allowing Urso to return to Brazil for personal reasons.[15] In three seasons, he made a total of 100 appearances in all competitions and scored 14 goals.
In July 2023, Urso rejoined Orlando City.[16] In December 2023, as part of the end of season roster moves, it was announced Urso had his option declined and was released as a free agent.[17]
Charlotte FC
On 21 February 2024, Urso signed with Charlotte FC ahead of their 2024 season.[18]
Personal life
Urso's brother is former professional footballer Peter de Almeida who retired in 2015 to become Junior's agent.[19]