He was introduced to entomology by Madame de Grostête-Tigny who was fascinated, like her husband, by chemistry and insects. Through her, Audinet-Serville met Pierre André Latreille (1762–1833). Latreille worked with him on the Dictionnaire des Insectes de l’Encyclopédie méthodique ("The Methodical Encyclopedia Dictionary of Insects").[2] Then, working with Guillaume-Antoine Olivier (1756–1814), he finished the book Faune française ("French Fauna") in 1830.
Audinet-Serville is particularly known for his work on the Orthoptera. He published, Revue méthodique de l’ordre des Orthoptères ("Methodical Review of the Order of Orthoptera") which appeared in Annales des sciences naturelles in 1831. Then, in 1839, in the series of works entitled les Suites à Buffon, a volume on the same order, Histoire naturelle des Insectes Orthoptères ("Natural History of Orthoptera Insects").