Plasmid pHT01 is generally stable in both B. subtilis and Escherichia coli, and can be used for protein expression in these host strains. pNDH33/pHT01 have been used to produce up to 16% of total protein output in B. subtilis. Pgrac100 is an improved version of Pgrac, which can produce up to 30% of total cellular proteins in B. subtilis.[3]
References
^Nguyen, H.D.; et al. (2007). "Expression Vectors for the Rapid Purification of Recombinant Proteins in Bacillus subtilis". Current Microbiology. 55 (2): 89–93. doi:10.1007/s00284-006-0419-5. PMID17624574. S2CID2299408.
^Titok, M. A.; et al. (2003). "Bacillus subtilis soil isolates: plasmid replicon analysis and construction of a new theta-replicating vector". Plasmid. 49 (1): 53–62. doi:10.1016/s0147-619x(02)00109-9. PMID12584001.
^Phan, T.P.T.; Nguyen, H.D.; Schumann, W. (2006). "Novel plasmid-based expression vectors for intra- and extracellular production of recombinant proteins in Bacillus subtilis". Protein Expression and Purification. 46 (2): 189–195. doi:10.1016/j.pep.2005.07.005. PMID16125412.