A total of 72 triathletes (36 per gender) will qualify to compete. A nation may enter a maximum of 6 triathletes (three per gender),with the exception of the winners of the 2021 Junior Pan American Games. The host nation (Chile) automatically qualified four athletes (two per gender). All other nations will qualify through various qualifying tournaments and rankings. A further three invitational slots, per gender, will also be awarded. A maximum five nations can enter the maximum of 3 triathletes in each gender.[1]
The top placed mixed relay team not yet qualified at the 2022 South American Games and 2023 Central American and Caribbean Games will each qualify two athletes per gender. The top five teams not qualified at the Pan American Mixed Relays Championship will also qualify two quotas per gender. The rest of the slots will be awarded through the ITU World Ranking as of July 10, 2023 and through wild card slots. An athlete cannot earn more than one slot for their country.
A country may enter the mixed relay competition if it has qualified at least two male and two female triathletes.