WASH complex subunit 2C is a protein that in humans is encoded by the WASHC2Cgene.[5] WASHC2C, also known as WASHCAP, VPEF, FAM21A, or FAM21C, is expressed ubiquitously.[6] WASHC2C is intracellular and is mainly in the cytosol associated with vesicles.[6] The protein has a low immune cell, human brain regional, and a low tissue specificity.[7] Some diseases that the protein are associated with are Vaccinia and Transient Tic Disorder.[8]
Function of WASHC2C
WASHC2C is part of the WASH core complex where it functions as a nucleation-promoting factor (NPF) at the surface of endosomes. Here it recruits and activates the Arp 2/3 complex to induce actin polymerization. Actin polymerization plays a key role in the fission of tubules that serve as transport intermediates during endosome sorting.[7]WASHC2C also mediates the recruitment of F-actin-capping protein dimer to the complex as well as the recruitment of the core complex to endosome membranes via binding to phospholipids. The strongest phospholipid bonding the protein does is with phosphatidylinositol 4-phosphate, phosphatidylinositol 3,5-biphosphate and phosphatidylinositol 5-phosphate.[7] GLUT1 5 is a protein that is recycled via the process of endosome-to-plasma membrane trafficking that WASHC2C plays an important role in .[7] The protein is also required for the endosomal recruitment of CCC, a multi-subunit protein complex, and other subunits such as COMMD1, VPS35L and CCDC93.[9]WASHC2C is involved in several other processes, negative regulation of barned-end actin filament capping, fluid base endocytosis endosomal transport, and regulation of substrate adhesion-dependent cell spreading.[9]
Structure
The WASHC2Cgene size is about 1320 amino acids with a molecular mass of 144911 daltons (Da) or 144.911 kilodaltons (kDa). The protein has a quaternary structure[10] and a basal isoelectric point of 4.66.[11]
Brill LM, Salomon AR, Ficarro SB, et al. (2004). "Robust phosphoproteomic profiling of tyrosine phosphorylation sites from human T cells using immobilized metal affinity chromatography and tandem mass spectrometry". Anal. Chem. 76 (10): 2763–72. doi:10.1021/ac035352d. PMID15144186.
Beausoleil SA, Villén J, Gerber SA, et al. (2006). "A probability-based approach for high-throughput protein phosphorylation analysis and site localization". Nat. Biotechnol. 24 (10): 1285–92. doi:10.1038/nbt1240. PMID16964243. S2CID14294292.
