RecBCD
| Eksodezoksiribonukleaza V | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 3.1.11.5 | ||||||||
| CAS broj | 37350-26-8 | ||||||||
| Baze podataka | |||||||||
| IntEnz | IntEnz pregled | ||||||||
| BRENDA | BRENDA pristup | ||||||||
| ExPASy | NiceZyme pregled | ||||||||
| KEGG | KEGG pristup | ||||||||
| MetaCyc | metabolički put | ||||||||
| PRIAM | profil | ||||||||
| Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
RecBCD (EC 3.1.11.5, Escherichia coli eksonukleaza V, E. coli eksonukleaza V, gen recBC endoenzim, RecBC dezoksiribonukleaza, gen recBC DNaza, eksonukleaza V, gen recBCD enzimi) je enzim E. coli bakterije koji inicira rekombinantnu popravku potencijalno letalnog prekida dvolančane DNK koji može da bude posledica izlaganju jonizirajućoj radijaciji, replikacionih grešaka, endonukleaza, oksidativnog oštećenja, i niza drugih faktora.[2] RecBCD enzim je helikaza koja odvija, ili razdvaja DNK lance, kao i nukleaza koja preseca jednolančanu DNK.[1] Ovaj enzim katalizuje sledeću hemijsku reakciju
- Eksonukleolitičko razlaganje (u prisustvu ATP) u bilo 5'- ka 3'- ili 3'- ka 5'- smeru čime se formiraju 5'-fosfooligonukleotidi
Struktura
Enzimski kompleks se sastoji od tri različite podjedinice: RecB, RecC, i RecD i stoga se kompleks naziva RecBCD. Pre otkrića recD gena,[3] ovaj enzim je bio poznat kao RecBC. Svaka podjedinica je kodirana zasebnim genom:
| gen | lanac | protein | funkcija |
|---|---|---|---|
| RecB | beta | P08394 | 3'-5' helikaza, nukleaza |
| RecC | gama | P07648 | prepoznaje Chi (engl. Crossover Hotspot Instigator) |
| RecD | alfa | P04993 | 5'-3' helikaza |
Reference
- ^ а б Singleton MR, Dillingham MS, Gaudier M, Kowalczykowski SC, Wigley DB (2004). „Crystal structure of RecBCD enzyme reveals a machine for processing DNA breaks”. Nature. 432 (7014): 187—93. PMID 15538360. doi:10.1038/nature02988.
- ^ Spies M, Kowalczykowski SC (2003). „Homologous recombination by RecBCD and RecF pathways”. Ур.: Higgins P. Bacterial Chromosomes. Washington, D.C: ASM Press. стр. 389—403. ISBN 978-1-55581-232-4.
- ^ Amundsen SK, Taylor AF, Chaudhury AM, Smith GR (1986). „recD: the gene for an essential third subunit of exonuclease V.”. Proc Natl Acad Sci U S A.. 83 (15): 5558—62. PMC 386327
. PMID 3526335. doi:10.1073/pnas.83.15.5558.
Literatura
- Spies M, Kowalczykowski SC (2003). „Homologous recombination by RecBCD and RecF pathways”. Ур.: Higgins P. Bacterial Chromosomes. Washington, D.C: ASM Press. стр. 389—403. ISBN 978-1-55581-232-4.
- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J.. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
Spoljašnje veze
- Exodeoxyribonuclease+V на US National Library of Medicine Medical Subject Headings (MeSH)
- Exodeoxyribonuclease+V,+E+coli на US National Library of Medicine Medical Subject Headings (MeSH)
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